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| Product Name | SPHK1a, Recombinant, Mouse, GST-Tag (Sphingosine kinase 1a) |
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| Description | Purity ≥ 50% by SDS-PAGE. Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1pmol of S1P/min. Assay Buffer: 50mM HEPES, pH 7.4, 150mM sodium chloride, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 4uM D-erythrosphingosine, and 0.1-0.5ug/ml sphingosine kinase 1. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
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| Size | 10ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: AF415213 |
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| Catalog # | S5455-01M |
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| Order / More Info | SPHK1a, Recombinant, Mouse, GST-Tag (Sphingosine kinase 1a) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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