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| Product Name | SPHK1, Recombinant, Human, full length (Sphingosine kinase 1) |
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| Description | Purity ≥80% by SDS-PAGE. Human Sphingosine kinase 1 (GenBank Accession No. AF200328), full length MW 46.9kD, expressed in an Baculovirus infected Sf9 cell expression system. Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D- erythro-sphingosine and [ -32P]ATP as substrates at pH 7.4 and 30 °C. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay buffer: 50mM HEPES, pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5 mM ATP, uM D-erythro-sphingosine, and 0.5ug/ml sphingosine kinase 1. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Stability: >6 months at -80 °C |
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| Size | 10ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: U33284 |
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| Catalog # | S5455-01 |
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| Order / More Info | SPHK1, Recombinant, Human, full length (Sphingosine kinase 1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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