产品名称 BpiI (BbvII) from UNITED STATES BIOLOGICAL
产品货号 B2640
产品价格 现货询价,电话:010-67529703
产品规格 200U, 1000U
产品品牌 UNITED STATES BIOLOGICAL
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Product NameBpiI (BbvII)
Description5'-G A A G A C (N)2?-3' 3'-C T T C T G (N)6^-5' Supplied With: R1625: Restriction Enzyme Buffer A, 10X, Dilute to 1X. 1X buffer compostion is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-02: Restriction Enzyme Buffer C, 10X, Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2, 50mM sodium chloride, 0.1mg/ml BSA. Unit Definition: One unit is defined as the amount of BpiI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of reaction buffer. Diluent Buffer: 10mM Tris-HCl, pH 7.4 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Enzyme Properties: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BpiI. Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 tes
Size200U, 1000U
Concentrationn/a
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Catalog #B2640
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