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| Product Name | Enteropeptidase/Enterokinase Activity Kit (Fluorometric) |
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| Description | Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence 'DDDDK' and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif. In The Enteropeptidase Activity Assay Kit, we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label 'AFC'. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enteropeptidase activity as low as 1 mU. |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | Measurement of Enteropeptidase activity in biological samples or purified. Enteropeptidase activity. Removing tag from recombinant proteins having recognition motif. |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K666 |
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| Order / More Info | Enteropeptidase/Enterokinase Activity Kit (Fluorometric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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