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| Product Name | Vitamin B12 (VB12), BioAssay™ ELISA Kit (Bovine) |
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| Description | Sample Type: Serum, plasma and tissue homogenates Intended Use: For the quantitative determination of endogenic bovine vitamin B12 (VB12) concentrations in serum, plasma and tissue homogenates. Sensitivity: 0.078pg/ml Range: 0.312-20pg/ml Specificity: This assay has high sensitivity and excellent specificity for detection of bovine VB12. No significant cross-reactivity or interference between bovine VB12 and analogues was observed. Test Principle: This BioAssay™ employs the direct competitive inhibition enzyme immunoassay technique. An antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added |
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| Size | 48 tests, 96 tests, 24 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 228017 |
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| Order / More Info | Vitamin B12 (VB12), BioAssay™ ELISA Kit (Bovine) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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