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| Product Name | RFP Expressing Liver Sinusoidal Microvascular Endothelial Cells, Human (T-25 flask) |
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| Description | Cell Culture Grade. Zeocin resistant RFP-HLSECs were selected and shipped in proliferating culture with a confluence of >90% (cells are provided at passage 4-5). ENDO-Growth medium containing 5% serum and growth supplement is recommended for culture. Cells have an average population doubling capacity of >16 when cultured. When you receive the cells, leave the flask in 37°C CO2 incubator for 1 hour. Then, replace the transport medium with fresh ENDO-Growth medium. Let the cells grow for 24 hours before subculture. Source: RFP Expressing Human Liver Sinusoidal Endothelial Cells (RFP-HLSECs), initiated by elutriation from dissociated normal human liver tissue and transfected with RFP- Lentiviral particles at passage 1. Cell Number: >90% confluent (>5x10e5 cells) in T25 flask Cell Characterization: Cytoplasmic VWF/Factor VIII: >95% positive by immunofluorescence Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence Cytoplasmic PECAM1: >95% positive by immunofluorescence RFP-HLSECs are negativ |
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| Size | T-25 flask |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 353389-T25 |
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| Order / More Info | RFP Expressing Liver Sinusoidal Microvascular Endothelial Cells, Human (T-25 flask) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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