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| Product Name | Flow Cytometry Staining Buffer (1X) |
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| Description | Flow Cytometry Staining Buffer is formulated and optimized for immunofluorescent staining of single-cell suspensions and subsequent dilution, suspension, washing, and storage of cells intended for flow cytometric acquisition and analysis. This product is supplemented with Bovine Serum Albumin (BSA) and the metabolic inhibitor sodium azide. Intended Use: This product is designed for flow cytometry applications with non-conjugated or fluorochrome-conjugated antibodies. Refer to product specification sheets to obtain the recommended working antibody dilutions. Surface Staining Protocol: 1. Wash cells twice with 2ml of Flow Cytometry Staining Buffer, spinning the tube at 300 x g for 5 minutes. 2. At this point, Fc receptor blocking reagents may be added to washed and counted cells. For antibodies directed to the Fc receptor follow the manufacturer's recommendations. If using excess pre-immune IgG to block Fc receptors, use 1ug of IgG per 1x10e5 cells to be stained. The excess IgG does not |
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| Size | 500ml |
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| Concentration | n/a |
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| Applications | FC |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | F4568-10 |
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| Order / More Info | Flow Cytometry Staining Buffer (1X) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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