| 产品详情 |
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| Product Name | BsuRI (HaeIII) |
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| Description | 5'-G G^C C-3' 3'-C C^G G-5' Concentration: 10u/ul Source: Bacillus subtilis R Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Thermal Inactivation: BsuRI is inactivated by incubation at 80°C for 20 minutes. Storage Buffer: 10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625-Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-04: Dilute to 1X for 100% BsuRI digestion. 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37°C Incubation Temperature: 37°C Unit Definition: One unit is defined as the amount of BsuRI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Methylation Effects: Dam, EcoKl,EcoBl: never overlaps - no effect. Dcm, CpG: may overlap - no effect. Protocol for Digestion: Add: Nuclease fr |
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| Size | 3000U |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | B3100 |
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| Order / More Info | BsuRI (HaeIII) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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