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| Product Name | Calpain I (Mu Calpain, Protein Kinase C Activating Factor) |
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| Description | Purity ≥ 90% as determined by reducing and non-reducing SDS-PAGE Coomassie. This protein consists of an 80kD large subunit and a 30kD small subunit. The protein was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns. Applications: This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: A- Dilute 1µl of the antiserum against µ-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000). B- Add 1µg of protein to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E-Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented. Stability: |
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| Size | 2ug, 5ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C1035-26Z |
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| Order / More Info | Calpain I (Mu Calpain, Protein Kinase C Activating Factor) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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