| 产品详情 |
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| Product Name | 488 Antibody labeling Kit (50 µg antibody per reaction) |
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| Description | ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes (Ex/Em=497 nm/525 nm) are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 488 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor™ 488 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 488 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 488 SE. |
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| Size | 2 labelings |
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| Concentration | n/a |
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| Applications | Biossay |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 1255 |
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| Order / More Info | 488 Antibody labeling Kit (50 µg antibody per reaction) from AAT BIOQUEST, INC. |
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| Product Specific References | n/a |
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