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Product Name | Beta-Lactamase Activity Assay Kit |
Description | Amplite™ Colorimetric Beta-Lactamase Activity Assay Kit. β-Lactamases are a large family of enzymes capable of hydrolyzing β-lactams. β-Lactam ring is the common element in all beta-lactam antibiotics including penicillin derivatives, cephalosporins, monobactams, and carbapenems. Through hydrolysis, β-lactamase breaks the β-lactam ring open, thus deactivates the molecule's antibacterial properties. Bacteria from clinical and non-clinical settings are becoming increasingly resistant to β-lactam antibiotics by synthesizing β-lactamase. To overcome this resistance, β-lactam antibiotics are often given with β-lactamase inhibitors such as clavulanic acid. Therefore, detection of β-lactamase activity is of central importance to assess beta-lactam antibiotics as well as to prevent antibiotics resistance. AAT Bioquest's Colorimetric Beta-Lactamase Activity Assay Kit offers a sensitive colorimetric assay for measuring β-lactamase activity in biological samples. The β-lactamase activity is detected using Nitrocefin, which changes color from yellow to red upon hydrolysis by β-lactamase. The assay can be performed using an absorbance microplate reader by measuring the OD ratio at the wavelength of 490 nm to 380 nm. |
Size | 200 tests |
Concentration | n/a |
Applications | Biossay, Colorimetric |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | 12551 |
Price | |
Order / More Info | Beta-Lactamase Activity Assay Kit from AAT BIOQUEST, INC. |
Product Specific References | n/a |
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