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| Product Name | GOT1, Recombinant, Human, aa1-413, His-tag |
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| Description | Purity ~95%(SDS-PAGE). Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Source: Recombinant protein corresponding to aa1-413 from human GOT1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~48.4kD (433aa) Specific Activity: >50units/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate/minute at pH 8.0 at 25°C. Activity Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 110mM Tris-HCl, pH 8.0, 0.44mM beta-nicotinamide adenine dinucleotide, reduced form, 55mM L-aspartate, 2.75mM alpha-ketoglutaric acid, 82.5units MDH. 2. Equilibrate to 25°C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 20ul of recombinant GOT1 prot |
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| Size | 20ug, 100ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | GOT1, Recombinant, Human, aa1-413, His-Tag (Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18) |
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| Gene, Accession, CAS # | Accession: NP_002070 |
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| Catalog # | 351255 |
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| Order / More Info | GOT1, Recombinant, Human, aa1-413, His-tag from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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