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| Product Name | Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1) |
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| Description | Purity > 300U/mg protein. Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) from Penicillium citrinum, a zinc dependent glyco-enzyme consisting of 270 amino acid residues, hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. Nuclease P1 is capable of hydrolyzing single stranded DNA and RNA completely to the level of mononucleoside 5'-monophosphates. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0. Activity: > 300U/mg protein using RNA substrate Unit Definition: One unit of nuclease activity is defined as the amount of enzyme that produces 1umole of acid soluble nucleotides from RNA based on E = 10,600 for RNA hydrolysates per minute at 37°C, pH 5.3. One unit of the enzyme liberates 1umole of orthophosphate from 3'-AMP per minute at 37°C, pH 7.2 Optimum Te |
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| Size | 100u, 250u |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | EC=3.1.30.1; Endonuclease P1; Nuclease 5'-Nucleotidehydrolase; 3'-Phosphohydrolase; NP1 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | N7000 |
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| Order / More Info | Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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