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Product Name | Caspase-3 Immunoassay Kit (Fluorometric) |
Description | Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT; Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected. |
Size | 100 assays |
Concentration | n/a |
Applications | Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | Kit-2305 |
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Order / More Info | Caspase-3 Immunoassay Kit (Fluorometric) from CREATIVE BIOMART |
Product Specific References | n/a |
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