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Product Name | Intact MMAF ADC ELISA Assay Kit |
Description | The Eagle Biosciences Intact MMAF ADC ELISA Assay Kit is designed, developed and produced for the quantitative measurement of antibody MMAF conjugate in serum or plasma. The assay utilizes the sandwich immunoassay technique with an antibody that binds to MMAF. Briefly, a mouse monoclonal antibody specific to MMAF is coated onto a microtiter plate. In the assay system, the assay calibrators, controls and test specimen are added to this microtiter plate. During the first incubation period, the anti-MMAF monoclonal antibody captures the MMAF-Antibody Conjugate of calibrators, controls and test samples. Unbound proteins are washed away with a wash step. A HRP (horseradish peroxidase) conjugate anti-human IgG tracer antibody is added to each well of the microtiter plate. After the second incubation, a ``sandwich'' immunocomplex of ``Anti-MMAF antibody - MMAF Antibody Conjugate - HRP-conjugated anti-human IgG antibody'' is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to MMAF Antibody Conjugate on the wall of the microtiter well is directly proportional to the amount of MMAF Antibody Conjugate level in the sample. Species Reactivity: human, mouse. Sample Types: serum, plasma. Range: 3.3 - 270 ng/mL. Sensitivity: 0.40 pg/mL. Time: 2 hours • The Eagle Biosciences Intact MMAF ADC ELISA Assay Kit is a highly sensitive ``sandwich'' test kit is intended for use in the quantitative determination of antibody MMAF conjugate level in serum or plasma of human, mouse, rat, primate, etc. in less than 2 hours. It is useful for pre-clinical and clinical pharmacology study of MMAF Antibody Drug Conjugate (ADC). The Eagle Biosciences Intact MMAF ADC ELISA Assay Kit is for research only and not used for diagnostic procedures. |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | MMAF ADC |
Gene, Accession, CAS # | n/a |
Catalog # | MMF91-K01 |
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Order / More Info | Intact MMAF ADC ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |
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