| 产品详情 |
| Edit |   |
|---|
| Product Name | Intact PTH ELISA |
|---|
| Description | Eagle Biosciences Intact PTH ELISA measures the concentration of PTH in its biologically active form of 84 amino acids (intact PTH). The assay uses two polyclonal antibodies directed against specific and different regions of human PTH; one antibody is biotinylated (and allows the binding of intact PTH to the microplate, through the binding of biotin with streptavidin coated in the wells of the microplate), the other antibody is linked to horseradish peroxidase (HRP) (and allows the detection of the signal derived from the molecules of captured intact PTH). This method has been set to avoid interference from inactive fragments of PTH: therefore, only the form of 84 amino acids is measured by the assay. From a procedural point of view, Calibrators, Controls and the serum samples are incubated with both antibodies simultaneously in the wells of the microplate. After a period of incubation (where intact PTH binds to the wells of the microplate through the bridge formed by the two antibodies), the microplate wells are washed with a wash solution to remove unbound material. The detection of the signal that quantifies the associated intact PTH is accomplished by incubation with TMB (tetramethylbenzidine), that reacts with the peroxidase and produces a colorimetric signal. This reaction is stopped by the addition of Stop Solution, a solution of sulphuric acid. The intensity of the color is directly proportional to the amount of intact PTH present in the wells; through a calibration curve, it is possible to determine the exact concentration of intact PTH bound. Sample Types: serum. Range: 7 - 210 pg/ml. Sensitivity: 0.49 pg/mL. Time: 3.5 hours • Parathyroid Hormone (PTH) is a hormone synthesized in the parathyroid gland in a 115 aminoacids form that, after post-translational modifications, reaches the biologically active form of 84 amino acids (Intact PTH). PTH is released in the blood, where is rapidly degraded (half-life about 4 minutes); intact PTH concentration in the blood is regulated by a negative feedback mechanism that involves calcium in serum. PTH is degraded inside the parathyroid glands, in liver, kidneys and bones. A high concentration of intact PTH in the blood may indicate kidney dysfunction. The measurement of the concentration of intact PTH in the blood is an effective way to diagnose the primary hyperparathyroidism and distinguish it from other diseases not related to parathyroid: in fact, intact PTH is elevated in 90% of cases of hyperparathyroidism. |
|---|
| Size | 1 x 96 well |
|---|
| Concentration | n/a |
|---|
| Applications | RUO |
|---|
| Other Names | Intact PTH |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | DCM157 |
|---|
| Price | |
|---|
| Order / More Info | Intact PTH ELISA from EAGLE BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|