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| Product Name | Anti-Mullerian Hormone ELISA Assay Kit |
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| Description | The Eagle Biosciences Human Anti-Muellerian Hormone ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human Anti-Muellerian Hormone in serum or heparin plasma samples. The assay utilizes the two-site ``sandwich'' technique with two selected antibodies that bind to different epitopes of human AMH.Assay calibrators, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to N-terminal AMH along with another AMH specific antibody labeled with horseradish peroxidase (HRP). After an initial incubation period, the plate is washed a ``sandwich'' of solid-phase antibody - human AMH-HRP-conjugated monoclonal antibody'' is formed. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human calprotectin in the test sample. A standard curve is generated by plotting the absorbance versus the respective human calprotectin concentration for each standard on a Cubic or point-to-point curve fitting. The concentration of human AMH in test samples is determined directly from this calibration curve. Sample Types: serum, plasma. Range: 0.11 - 20 ng/mL. Sensitivity: 0.02 ng/mL. Time: 4.5 hours • Anti-Muellerian Hormone (AMH) or Muellerian-inhibiting hormone (MIH) is a glycoprotein hormone structurally related to inhibin and activin from the transforming growth factor beta superfamily, whose key roles are in growth differentiation and folliculogenesis. AMH expression is critical to sex differentiation at a specific time during fetal development, and appears to be tightly regulated by nuclear receptor SF1, transcription GATA factors, sex-reversal gene DAX1, and follicle-stimulating hormone (FSH). AMH is activated by SOX9 in the Sertoli cells of the male fetus thereby arresting the development of fallopian tubes, uterus, and upper vagina. AMH is also a product of granulosa cells of the preantral and small antral follicles in women. As such, AMH is only present in the ovary until menopause. AMH can serve as a molecular biomarker for relative size of the ovarian reserve and can also be used as a marker for ovarian dysfunction, such as in women with polycystic ovary syndrome (PCOS). |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Anti-Mullerian Hormone |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | AMH31-K01 |
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| Order / More Info | Anti-Mullerian Hormone ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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