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Product Name | Malaria ELISA Kit |
Description | The Malaria ELISA is intended for the qualitative determination of IgG/IgM antibodies against Plasmodium in human serum or plasma (citrate, heparin). This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader. |
Size | 96 wells |
Concentration | n/a |
Applications | RUO |
Other Names | EIA, enzyme immunoassay, assay |
Gene, Accession, CAS # | n/a |
Catalog # | EIA5511R |
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Order / More Info | Malaria ELISA Kit from DRG INTERNATIONAL INC. |
Product Specific References | n/a |
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