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Product Name | Human IGF-I, Biotin labeled (Biotin-IGF-I, human) |
Description | Product Specifications Biotin-IGF-I, human: Biotin- IGF-I is prepared by a selective biotinylation procedure, that ensures full binding capacity to IGF-binding proteins-1 to -5. As in natural IGF-I, binding to IGFBP-6 is strongly reduced. Binding to antiserum PAA is not affected.Examples of applications of biotinylated IGF's can be seen in application note 4.Molecular Weight 8005.5 Da.Formulation: Lyophilised from a solution of 0.5 ml TBS, pH 7.4, containing 10 µg of the biotinylated product . A preservative and a stabilising protein of non-mammalian origin are added to ensure stability of the reconstituted product.Recommended dilution: 1: 100 to 1: 250 for western ligand blotting. Tested with our western-ligand blotting kit, in ELISA, in cross-linking experiments, in IHC and binding studies. The use of biotinylated IGF-I in experimental celltheraphy has demonstrated, that it is biologically active in-vitro and in vivo. Storage: Store refrigerated upon arrival. The product is stable at 4 °C for at least one year. Reconstitute with 0.5 ml water and store aliquots frozen at - 20 °C. Avoid repeated freeze-thawing cycles. |
Size | 10 µg |
Concentration | n/a |
Applications | n/a |
Other Names | Biotin-IGF-1, Insulin-like Growth Factor-1, human, Biotinylated |
Gene, Accession, CAS # | n/a |
Catalog # | BIO-IGF1-10 BIO-IGF1-20 |
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Order / More Info | Human IGF-I, Biotin labeled (Biotin-IGF-I, human) from IMMUNOLOGICAL & BIOCHEMICAL TESTSYSTEMS GmbH |
Product Specific References | Michael E. Davis*, Patrick C. H. Hsieh*, Tomosaburo Takahashi*, Qing Song, Shuguang Zhang, Roger D. Kamm, Alan J. Grodzinsky, Piero Anversa, and Richard T. Lee*: Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction. PNAS | May 23, 2006 | vol. 103 | no. 21 | 8155-8160 Full text of the article: www.pnas.org/cgi/content/full/103/21/8155- Altmann et. al.: The relationships between leptin concentrations and body fat reserves in lambs are reduced by short-term fasting.Journal of Animal Physiology and Animal Nutrition 2006, Vol. 90, issue 9-10, p407 - 413. |
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