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Product Name | Rat IGF-I, recombinant protein (Receptor Grade) |
Description | Product Specifications: The Product: Rat IGF-I is a polypeptide comprising of 70 amino acids, three of which differ from the amino acid sequence of human, IGF-I. Rat IGF-I has been shown to stimulate growth in rat L6 myoblasts and other cell lines. Source: Rat IGF-I is produced recombinantly in E.coli using a patented expression system. The rat IGF-I molecule is correctly folded and several chromatography purification steps are then employed to yield Receptor Grade rat IGF-I. Purity: > 95% (as determined by HPLC and N-terminal sequence analysis) Molecular Weight: 7687 daltons - confirmed by Mass Spectrometry N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence Biological Activity:(stimulation of protein synthesis in rat L6 myoblasts) ED50 <30 ng/ml Endotoxin (LAL): <0.1 EU/ug Stability: at least 2 years at 2-4°C (lyophilised) Appearance: White Powder State: Dried from 0.1 M acetic acid: under dry nitrogen @ slight vacuum (-25kPa) Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. <1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles. |
Size | 20 µg |
Concentration | n/a |
Applications | n/a |
Other Names | Igf-1, insulin-like growth factor-1, Somatomedin c, ILGF1, Mechano growth factor |
Gene, Accession, CAS # | n/a |
Catalog # | RU020 RU100 RM010 |
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Order / More Info | Rat IGF-I, recombinant protein (Receptor Grade) from IMMUNOLOGICAL & BIOCHEMICAL TESTSYSTEMS GmbH |
Product Specific References | Ciucci et al.: Insulin-Like Growth Factor 1 (IGF-1) Mediates the Effects of Enriched Environment (EE) on Visual Cortical DevelopmentPLoS ONE. 2007 |
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