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| Product Name | PDIA4, Recombinant, Human, aa21-645, His-Tag |
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| Description | Purity ~90% (SDS-PAGE). PDIA4, also known as ERP72, is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. It is involved in the catalysis of protein- S-S-bond rearrangement. PDIA3 and PDIA4 act as proteases, protein disulfide isomerases, phospholipases or a combination of these. Source: Recombinant protein corresponding to aa21-645 from human PDIA4, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~72.9kD (646aa) Specific Activity: >10 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT. Activity Assay: 1. Prepare a 180ul assay buffer into a suitable container: The concentrations are 0.67mM DTT, insulin 5.3mg, 1.2mM EDTA 2. Dilute insulin 8mg/ml in 3mM EDTA, pH 7.0, and incubate diluted insulin for 5 minutes at 37°C. 3. Dilute PDIA4 to 800ug/ml in 0.1% BSA. AA |
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| Size | 10ug, 50ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | PDIA4, Recombinant, Human, aa21-645, His-Tag(Protein disulfide-isomerase A4 , Endoplasmic reticulum resident protein 72, ERP70, ERP72) |
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| Gene, Accession, CAS # | Accession: NP_004902 |
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| Catalog # | 351418 |
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| Order / More Info | PDIA4, Recombinant, Human, aa21-645, His-Tag from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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