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Antigenic Specificity | Lactate Dehydrogenase |
Clone | polyclonal |
Host Species | Goat |
Reactive Species | rabbit |
Isotype | n/a |
Format | IgG fraction |
Size | 100 µg |
Concentration | 1.0 mg/ml |
Applications | ELISA, Immunoprecipitation, Western Blot. LACTATE DEHYDROGENASE antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-LACTATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species. |
Immunogen | This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle. |
Other Names | goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M |
Gene, Accession # | LDH-A, Gene ID: 100009107, NCBI: 126050, UniProt: P13491 |
Catalog # | 200-1173-0100 |
Price | |
Order / More Info | Lactate Dehydrogenase Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Zaman K, et al. (1999) Protection from oxidative stress-induced apoptosis in cortical neuronal cultures by iron chelators is associated with enhanced DNA binding of hypoxia-inducible factor-1 and ATF-1/CREB and increased expression of glycolytic enzymes, p21(waf1/cip1), and erythropoietin. J Neurosci 19(22):9821-9830. |