产品详情 |
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Antigenic Specificity | Akt phospho S473 , internal |
Clone | 17F6.B11 |
Host Species | Mouse |
Reactive Species | human, mouse, rat, monkey |
Isotype | IgG1 kappa |
Format | ATTO 594 conjugate |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, FLISA, Flow Cytometry, IF Microscopy, Western Blot. Anti-AKT pS473 Antibody ATTO 594 Conjugated is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined. |
Immunogen | Akt phospho S473 ATTO594 Conjugated Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein. |
Other Names | mouse anti-AKT pS473 ATTO 594 conjugated Antibody, ATTO 594 conjugated mouse anti-AKT pS473 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, AT594, ATTO 594, ATTO-TEC 594 |
Gene, Accession # | AKT1, Gene ID: 207, NCBI: 62241011, UniProt: P31749 |
Catalog # | 200-355-268 |
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Order / More Info | Akt phospho S473 , internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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