产品详情 |
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Antigenic Specificity | Akt phospho S473 |
Clone | 17F6.B11 |
Host Species | Mouse |
Reactive Species | human, mouse, rat, monkey |
Isotype | IgG1 kappa |
Format | DyLight™ 649 conjugate |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, FLISA, Flow Cytometry, IF Microscopy, Western Blot. This monoclonal antibody is suitable for immunofluorescence microscopy, FLISA and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined. |
Immunogen | This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein. |
Other Names | mouse anti-AKT pS473 DyLight™649 conjugated Antibody, DyLight649 conjugated mouse anti-AKT pS473 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1 |
Gene, Accession # | AKT1, Gene ID: 207, NCBI: 62241011, UniProt: P31749 |
Catalog # | 200-343-268 |
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Order / More Info | Akt phospho S473 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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