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| Product Name | 6-Phosphogluconic Acid (6-PGA) Assay Kit (Colorimetric) |
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| Description | 6-Phosphogluconate (6-PGA) is an intermediate of both Pentose Phosphate Pathway (PPP) and Entner-Doudoroff Pathway. It is produced by the hydrolysis of 6-Phosphogluconolactone, catalyzed by 6-Phosphogluconolactonase. In the Pentose Phosphate Pathway, 6-PGA is utilized by 6-Phosphogluconate Dehydrogenase to generate ribulose-5-Phosphate and NADPH. These products are important for nucleic acid synthesis and various anabolic processes. In Prokaryotes, 6-Phosphogluconate is the main metabolite of Entner-Doudoroff pathway, and is converted into Pyruvate using both 6-Phosphogluconate Dehydratase and 2-Keto-3-Deoxyphosphogluconate aldolase. Recent studies show that long-term exposure to glucose perturbs the Pentose Phosphate Pathway, causes significant accumulation of 6-Phosphogluconate and impairs beta cell function. Measurement of 6-Phosphogluconate levels therefore is important for evaluating Pentose Phosphate Pathway, developing therapeutic approaches for diabetes research, and analyzing the Entner-Doudoroff Pathway in bacteria. The 6-Phosphogluconate assay kit can be used with a variety of sample types. In this assay, 6-Phosphogluconate is converted to Ribulose-5-Phosphate by 6-Phosphogluconate Dehydrogenase in the presence of NAD, to form NADH, which reduces a probe and generates strong absorbance at 450 nm. This 6-Phosphogluconate Assay Kit is simple, sensitive & easy to use and can detect 6-Phosphogluconate levels lower than 20 µM. Sample types: adherent cells, heart, hela cells, jurkat cells, kidney, liver, suspension cells, tissues |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | Measurement of 6-Phosphogluconic Acid in various tissues/cells. Analysis of Pentose Phosphate Pathway and Entner-Doudoroff Pathway. |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K380 |
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| Order / More Info | 6-Phosphogluconic Acid (6-PGA) Assay Kit (Colorimetric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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