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| Product Name | Mouse- / Rat-IGFBP-2 ELISA |
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| Description | The Eagle Biosciences Mouse/Rat IGFBP-2 ELISA, is a so-called sandwich-assay. It utilizes two different specific high affinity polyclonal antibodies for this protein. The IGFBP-2 in the samples binds quantitatively to the immobilized antibody. In the following step, the biotinylated antibody in turn binds IGFBP-2. After washing, a streptavidin-peroxidase-enzyme conjugate will be added, which will bind highly specific to the biotin of the antibody. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the IGFBP-2 content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption. Species Reactivity: mouse, rat. Sample Types: serum, plasma. Range: 0.01 - 200 ng/mL. Sensitivity: 0.01 ng/mL. Time: 3 hours • Insulin-like growth factors (IGFs) regulate the proliferation, differentation, apoptosis, cell adhesion and metabolism in various tissues and cell types IGFBP-2 is an unglycosylated polypetide of 31.3 kDa, which forms binary IGF-complexes and shows no circadian rhythm in the circulation. The serum concentration of IGFBP-2 increases in fasting, after major surgery and after trauma, but the increasing of the concentration is most intensive in malignant diseases. The correlation of the IGFBP-2 level to the degree of progression is a striking feature in various tumor types as is the normalization of the IGFBP-serum levels after remission (5-10). During the GH-therapy, e.g. in short stature and in GH-abuse (doping) the IGFBP-2 level decreases. In Trisomy 18 IGFBP-2 in maternal serum is decreased and IGFBP-1 is increased; therefore the ratio IGFBP-2 /IGFBP-1 is a marker for this chromosome abnormality (17). Transgenic organisms are a good opportunity to investigate the function of genes or proteins. The mouse or rat model is a well-suited system for investigation of the relevance of IGFBP-2 in physiological and pathological processes. Over expression of the IGFBP-2 gene in mice results in a weight reduction of 30% in spleen and moderately reduced weight in other organs (18). Effects of IGFBP-2 on the organism can be compensated through the modified expression of other IGF Binding proteins. Especially in tumor biology the mouse and rat systems enable investigation of the systemic relevance of IGFBP-2. IGFBP-2 influences tumor cells as it induces catalase activity in adrenocortical cells (19). Furthermore IGFBP-2 interacts with tumor cells via its RGD-amino acid sequence and seems to stimulate cell invasion of glioma cells (20). |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | E08 Insulin like growth factor IGFBP-2, IGF-II, IGFBP ASSAY EIA ELISA GROWTH IGFBP-2 IMMUNOASSAY INSULIN INSULIN-LIKE KIT mouse rat murine protein |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | E08 |
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| Order / More Info | Mouse- / Rat-IGFBP-2 ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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