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| Product Name | RBM3 ELISA Assay Kit |
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| Description | The Eagle Biosciences Human RBM3 ELISA (Enzyme-Linked Immunosorbent Assay) Assay Kit is an immunological quantitative detection method based on a sandwich ELISA. The Eagle Biosciences Human RBM3 ELISA Assay Kit is performed in a 96-well microplate. The wells of the microplate are pre-coated with a specific capture antibody against human RBM3 that binds free RBM3 from the patient sample. In one step simultaneous incubation of the RBM3 standards or samples and of the detection conjugate (peroxidase conjugate of an anti-RBM3 antibody) is carried out. Incubation takes place 2 hours at 20-25°C. After a washing step, the colour substrate TMB (tetramethylbenzidine) is added to the wells. The reaction is stopped by adding the Stop Solution and the yellow colour is read in a microtiter plate reader at 450 nm. The concentration of RBM3 in a sample is determined by interpolation from the standard curve. Sample Types: serum, plasma. Range: 31-2000 pg/ml. Sensitivity: 10 pg/ml. Time: 2.5 hours • The RNA-binding protein 3 (RBM3) is a member of the glycine-rich RNA-binding protein family. The exact role of the cold shock protein RBM3 is not yet clear [2, 6]. The role of mRNA-binding proteins can be attributed to a regulation of mRNA splicing, stability, transport and ultimately the translation of mRNA into proteins. RBM3 consists of 157 amino acids with a predicted mass of 17 kD (1, 2) and is one of the few proteins that are upregulated at low temperature [3-5]. It is known that RBM3 acts especially in cellular stress (eg. hypothermia) as mRNA chaperones and protein translation stabilizer [1, 4, 5]. Chip et al. were able to show that RBM3 has anti-apoptotic properties in neurons [7] and recent studies show that RBM3 mediates structural plasticity and protective effects of neurodegeneration (8). For these reasons, RBM3 may play an important role as biomarker for many neurodegenerative diseases and could be used to monitor the effectiveness of therapeutic hypothermia. Several studies report RBM3 as prognostic marker in cancer or in linking stress-regulated RNA splicing to tumorgenesis. Low nuclear expression of RBM3 has been found to be associated with poor prognosis in several cancer forms e.g. breast, ovarian, colorectal, prostate cancer and malignant melanoma (9-14). |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | RNA-binding protein 3, RBM3 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | BTRBM-001 |
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| Order / More Info | RBM3 ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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