| 产品详情 |
| Edit |   |
|---|
| Product Name | CoproELISA Entamoeba |
|---|
| Description | Plates are coated with specific polyclonal antibodies directed against Entamoeba antigens. Fecal sample to be tested is diluted in stool diluent and incubated with the pre-coated plate. In this step Entamoeba antigens are bound to the immobilized antibodies. Non-specific antigens are removed by washing. Anti- Entamoeba monoclonal antibody conjugated to horseradish peroxidase (HRP) is added and incubated. In this step the HRP-conjugate is bound to the pre-bound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm. The absorbance is proportional to the number of Entamoeba cells present in the sample. Sample Types: stool. Range: Cut-Off. Sensitivity: Cut-Off. Time: 2 hours • Entamoeba histolytica/dispar (E. histolytica/dispar) are intestinal protozoan parasites that are transferred via the faecal-oral route and infect up to 10 % of the human population (1). Entamoeba infections are most common in the developing world and are associated with poor sanitation where the Entamoeba cysts are transmitted from contaminated food and water. After ingestion trophozoites the Entamoeba vegetative form are released from their cyst capsules into the small intestine. Trophozoites which survive strictly in the host intestine and fresh stool migrate to the large intestine where they live and multiply by binary fission. Trophozoites of E. dispar are noninvasive and reside in the intestine while those of E. histolytica can invade the intestinal mucosa causing human amoebiasis (1 and 2). Symptoms of amoebiasis can range from mild diarrhea to dysentery with blood and mucus in the stool. While most E. histolytica infected people remain asymptomatic, without proper treatment, this disease can lead to severe complications, including abscess of vital organs, most commonly in the liver, and in some cases in the lungs, and the brain (3). It is estimated that E. histolytica, represents 10% of human Entamoeba infections which cause the death of between 40,000 to 100,000 people every year (4). It is, therefore, important to diagnose the infection and provide appropriate treatment. The most common treatment for amoebiasis is the administration of antibiotics, such as metronidazole and tinidazole. |
|---|
| Size | 1 x 96 well |
|---|
| Concentration | n/a |
|---|
| Applications | RUO |
|---|
| Other Names | Entamoeba, stool, fecal |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | 754-01 |
|---|
| Price | |
|---|
| Order / More Info | CoproELISA Entamoeba from EAGLE BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|