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Product Name | Human IL-8 ELISA KIT |
Description | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-8 is added to the wells and binds to the combination of capture antibody-IL-8 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-8 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-8 standard dilutions and IL-8 sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 31.25 - 1000 pg/ml. Sensitivity: 4 pg/ml. Time: 3.5 hours • The Eagle Biosciences Human Interleukin 8 (IL-8) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 8 (IL-8) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human Interleukin 8 (IL-8) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Interleukin 8, IL-8 |
Gene, Accession, CAS # | n/a |
Catalog # | IL831-K01 |
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Order / More Info | Human IL-8 ELISA KIT from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |
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