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| Product Name | NMNAT1, Recombinant, Human, aa115-270, His-Tag (Nicotinamide nucleotide adenylyltransferase 1, NMNAT, PNAT1) |
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| Description | Purity ~95% (SDS-PAGE). NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Source: Recombinant protein corresponding to aa115-270 from human NMNAT1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~36kD(315aa) Biological Activity: Specific activity is >7000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP/minute at pH 8.0 at 37°C. Activity Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 50mM Tris-HCl (pH 8.0), 50mM semicarbazide, 22mM magnesium chloride (MgCl2), 2.2mM beta-NMN, 2.2mM ATP, 5.5unit ADH, 2.2% (v/v) ethanol. |
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| Size | 20ug, 100ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: NP_073624 |
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| Catalog # | 351397 |
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| Order / More Info | NMNAT1, Recombinant, Human, aa115-270, His-Tag (Nicotinamide nucleotide adenylyltransferase 1, NMNAT, PNAT1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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