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| Product Name | Troponin I ELISA Kit |
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| Description | An enzyme immunoassay for the quantitative determination of caridac specific troponin I in serum. This test is intended for the use with serum. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The cTnI ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes four unique monoclonal antibodies directed against distinct antigenic determinants on the molecule. Three mouse monoclonal anti-troponin I antibodies are used for solid phase immobilization (on the microtiter wells). The fourth antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the four antibodies, resulting in the troponin I molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 90-minute incubation at room temperature, the wells are washed with water to remove unbound-labeled antibodies. A solution of tetramethylbenzidine (TMB) Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of 1N hydrochloric acid (HCl) changing the color to yellow. The concentration of troponin I is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. |
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| Size | 96 wells |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | EIA, enzyme immunoassay, assay |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | EIA2952R |
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| Order / More Info | Troponin I ELISA Kit from DRG INTERNATIONAL INC. |
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| Product Specific References | n/a |
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