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| Product Name | Smad Ubiquitination Regulatory Factor-1, N-terminal FLAG-Tag, Recombinant, Human |
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| Description | Purified ≥80%. Source: Recombinant corresponding to aa150-end of human SMURF 1 with N-terminal FLAG tag expressed in a Baculovirus infected Sf9 cell expression system (NM_020429). Specific Activity: 0.0055pmol/min/ug Assay conditions: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5ug), E2 (2.8ug), Biotin-Ubiquitin (0.5ug)and Smurf1 (0-1ug) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against Smurf1, incubate 1 hour. Finally, incubate 1 hour with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (lambda-exc=330 nm, lambda-em=620 nm). Applications: Suitable for use in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors and selectivity profiling. |
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| Size | 20ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | Smad Ubiquitination Regulatory Factor-1, N-terminal FLAG-Tag, Recombinant, Human (Smad Ubiquitination Regulatory Factor 1, Smurf1, E3 Ubiquitin Ligase SMURF1, HGNC:16807, KIAA1625, Smad-specific E3 Ubiquitin Ligase 1, SMAD-specific E3 Ubiquitin Protein Ligase 1) |
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| Gene, Accession, CAS # | Accession: NM_020429 |
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| Catalog # | S1014-60R |
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| Order / More Info | Smad Ubiquitination Regulatory Factor-1, N-terminal FLAG-Tag, Recombinant, Human from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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