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| Product Name | P300 Homogeneous BioAssay™ Kit |
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| Description | The p300 Homogeneous BioAssay™ Kit contains an AlphaLISA® assay designed for screening inhibitors of p300 in a convenient 384-well format. This efficient method requires no time-consuming washing steps. Only a few simple steps on a microtiter plate are needed. The assay is based on the enzymatic transfer of an acetyl group from acetyl CoA to a specific lysine residue within a biotinylated peptide substrate. After the p300 reaction, the sample is incubated with a combination of an antibody that specifically binds the acetylated peptide and acceptor beads that bind the antibody. This is followed by incubation with streptavidin-labeled donor beads and reading of the Alpha-counts. Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Components: P300 human recombinant enzyme, 1x1ug Acetyl CoA (1mM), 1x500ul 3X HAT assay buffer, 1x4ml Biotinylated H3 Peptide Substrate, 1x700ul Primary antibody 20, 1x40ul |
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| Size | 1 kit |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 171940 |
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| Price | |
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| Order / More Info | P300 Homogeneous BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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