| 产品详情 |
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| Antigenic Specificity | 5-mC / 5-Methylcytosine |
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| Clone | polyclonal |
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| Host Species | Sheep |
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| Reactive Species | broad, rat |
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| Isotype | n/a |
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| Format | azide-free unconjugated |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | IF, IHC, IHC-Fr (10 µg/ml), IHC-P (10 µg/ml), IP (25 - 100 µg/ml), WB (1:1000) |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | 5-mC / 5-Methylcytosine Antibody (Azide-free) LS-C187857. Specificity: Sheep Anti 5-Methylcytosine (5-MeC) recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5. This modification alters the structure of cytosine but not its base-pairing properties. The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination. For example, in mammals, methylation occurring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, whereas in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements. The deamination of 5-MeC results in the formation of thymine (T) instead of uracil. Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations. The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites. Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers. Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers. Sheep Anti-5-MeC is reported as suitable for use in immunocytochemistry. |
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| Immunogen | Keyhole limpet hemocyanin (KLH) conjugated 5-methylcytosine. |
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| Other Names | n/a |
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| Gene, Accession # | n/a |
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| Catalog # | LS-C187857 |
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| Price | |
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| Order / More Info | 5-mC / 5-Methylcytosine Antibody from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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