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| Product Name | HE+ Pervanadate Lysate |
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| Description | Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. Human aortic endothelial cells treated with pervanadate for 30 minutes display a significant increase in tyrosine phosphorylation as shown by western blotting using anti-Phosphotyrosine. Confluent cultures of human aortic endothelial cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1 mM) for 30 minutes at 37°C. Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer. Applications: SDS-PAGE/Western blotting 20ml/well. End user should determine optimal quantity for their particular applications and experiments. |
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| Size | 100ug |
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| Concentration | n/a |
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| Applications | WB |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | H1833A |
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| Order / More Info | HE+ Pervanadate Lysate from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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