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| Product Name | Phosphoglycerate Dehydrogenase (PHGDH) Activity Kit (Color) |
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| Description | Phosphoglycerate Dehydrogenase (PHGDH: EC 1.1.1.95) is the first enzyme that participates in the L-serine synthesis pathway. PHGDH catalyzes the reversible reaction that converts 3-phosphoglycerate into 3-phosphohydroxyglycerate and the NADH-dependent reduction of a-ketoglutarate into D-2-hydroxyglutarate (D-2HG). In humans, PHGDH deficiency causes severe health problems including congenital microcephaly, psychomotor retardation, and intractable seizure. Recent studies have shown increased PHGDH activity in a wide variety of cancers, particularly in breast and melanoma tumors. Therefore, the measurement of PHGDH activity has become of great importance in searching potential therapeutic agents against cancer. The Phosphoglycerate Dehydrogenase Assay kit provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, Phosphoglycerate Dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal (OD: 450 nm). The assay is simple, sensitive, high-throughput adaptable and can detect Phosphoglycerate Dehydrogenase activity less than 0.1 mU per sample. Sample types: adherent cells, animal tissues, liver, kidney, suspension cells |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | Measurement of PHGDH activity in purified/crude enzyme preparations and biological samples such as cell/tissue lysates |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K574 |
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| Order / More Info | Phosphoglycerate Dehydrogenase (PHGDH) Activity Kit (Color) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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