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| Product Name | Mung Bean Nuclease |
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| Description | Mung Bean Nuclease belongs to a class of enzymes that demonstrate a preference for single-stranded nucleic acids, lack sugar specificity and hydrolyze single-stranded substrates to products with 5'-phosphoryl and 3'-hydroxyl termini. Mung Bean Nuclease catalyzes the degradation of single-stranded DNA and RNA endonucleolytically to yield 5'-phosphoryl terminated products. While the nuclease prefers ssDNA over dsDNA by 30,000-fold, at very high concentrations, such as 14u/ug of DNA, the enzyme degrades double-stranded DNA preferentially from both ends (1,2,3). Unit Definition: One unit is defined as the amount of enzyme required to produce 1ug of acid-soluble nucleotides per minute at 37°C in 30mM sodium acetate, pH 5.0, 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA, 5% glycerol. Additional Information Molecular Weight: 39kD Requirement: Zn2+. Inhibitors: Mung Bean Nuclease is inhibited by high salt concentrations (80-90% inhibition in 200-400mM NaCl). Use with 0.001% Triton® |
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| Size | 2000U |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | M9218 |
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| Price | |
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| Order / More Info | Mung Bean Nuclease from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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