| 产品详情 |
| Edit |   |
|---|
| Product Name | Luminometric Calcium Assay Kit |
|---|
| Description | Screen Quest™ Luminometric Calcium Assay Kit. Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. Our coelenterazine-based kit is much more sensitive than the fluorescence-based calcium assay kits (such as Fluo-4, Fluo-3, Calcium-3 and Calcium-4). This kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring of intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues. |
|---|
| Size | 100 plates |
|---|
| Concentration | n/a |
|---|
| Applications | Biossay |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | 36306 |
|---|
| Price | |
|---|
| Order / More Info | Luminometric Calcium Assay Kit from AAT BIOQUEST, INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|