| 产品详情 |
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| Antigenic Specificity | Green Fluorescent Protein (GFP) |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | Aequorea victoria |
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| Isotype | n/a |
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| Format | Protein A purified |
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| Size | 0.1 mg |
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| Concentration | n/a |
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| Applications | Immunocytochemistry (ICC),Immunoprecipitation (IP),Western Blotting (WB) |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ,=509 nm) when excited (excitation by Blue or UV light, absorption peak λ,=395 nm).GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties.Antibodies raised against full-length GFP variants should also detect other variants of the protein. |
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| Immunogen | EGFP, a native full-length protein |
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| Other Names | n/a |
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| Gene, Accession # | n/a |
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| Catalog # | ABIN302066 |
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| Price | |
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| Order / More Info | Green Fluorescent Protein (GFP) Antibody from ANTIBODIES-ONLINE GmbH |
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| Product Specific References | PubMed: 20510892, 16724116 |
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