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Antigenic Specificity | proteoglycan, hyaluronic acid binding region; aggrecan |
Clone | 12/21/1-C-6 |
Host Species | Mouse |
Reactive Species | bovine, canine, chicken, fish, human, porcine, rat |
Isotype | IgG1 |
Format | supernatant |
Size | 1.0 ml |
Concentration | n/a |
Applications | Immunohistochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | To expose the core protein epitope, the tissue must be reduced and alkylated. Treat the tissue with 10mM dithiothreitol in TBS pH 7.4 for 2 hr at 37°C for 2 hr. Follow by alkalation with 40 mM iodoacetamide in PBS for 2 hr. The amount of antigent used in immunization was injected at six sites with 0.5 ml of protein (200-800 µg protein/ml) and 0.5 ml complete Freund's adjuvant on day 1; 0.5 ml Freund's incomplete adjuvant on day 3; 0.5 ml Dulbecco's PBS on days 6, 9, and 12; on day 14. The mouse was sacrificed for the route of immunization. |
Immunogen | reduced and alkylated rat chondrosarcoma proteoglycan monomer |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | 12/21/1-C-6 |
Price | |
Order / More Info | proteoglycan, hyaluronic acid binding region; aggrecan Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK |
Product Specific References | PubMed: 1384430, 26364138, 27699833, 26364138, 10486511, 11474365, 11493848, 11273046, 11980843, 14594208, 14699636, 23313125 |
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