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| Product Name | RSK1, active, Recombinant, Human |
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| Description | Purity ~94% by SDS-PAGE and Coomassie blue staining. N-terminal His6 tagged recombinant full-length human RSK1 expressed in Sf21 cells. Activated with PDK1 and MAP Kinase 2/Erk2 and repurified using glutathione-Sepharose, heparin-Sepharose and Ni2+/NTA agarose. Specific Activity: ~2900U/mg, Unit Definition: One unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM M2355-09 MAPKAP Kinase 2 Substrate Peptide (KKLNRTLSVA) per minute at 30°C with a final ATP concentration of 100uM. Identity Confirmation: MS Tryptic Fingerprint: Confirmed identity as RSK1 with 38% amino acid coverage of the translated sequence. Applications: Kinase Assay: Kinase Assay: 0.3-2.5ng phosphorylated 30uM MAPKAP Kinase 2 Substrate Peptide in the assay. Assay background was subtracted from the actual counts to yield the results |
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| Size | 5ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | RSK1, active, Recombinant, Human (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Protein S6 Kinase alpha-1, RPS6KA1, S6K-alpha-1) |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | R9399-10 |
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| Order / More Info | RSK1, active, Recombinant, Human from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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