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| Product Name | DNA Polymerase I Klenow Fragment, Exo-Minus, Recombinant |
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| Description | Klenow fragment, exo, is an N-terminal truncation of E. coli DNA Polymerase I at amino acid #323. In this construct, the domain containing the 5?-3? exonuclease activity is deleted, leaving the polymerase function intact. In addition two mutations are introduced (D355A and E357A) which abolish the 3?-5? exonuclease activity. The enzyme features strand displacement capability , an error rate of at 100x100e-6 bases and has no intrinsic exonuclease activity. • Recombinant single protein; not a fusion construct. • Generates probes using direct or indirect labeling methods. • Specific Activity: 15,000-20,000 units/mg. • Low DNA content • High concentration, automation grade. • Supplied with 10X Reaction Buffer. High concentrations supplied with 1X Dilution Buffer Size1 100ul 5u/ul Size2 50ul 40u/ul Size3 20ul 200u/ul Also included are 1ml dilution buffer and 2ml 10X reaction buffer. Application(s): Dideoxy sequencing (2), blunt ending restriction fragments, second strand cDNA synthesis for |
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| Size | 500U, 2000U, 4000U |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | EC=2.7.7.7; E. coliSupplied with Reaction Buffer |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | D3941-01 |
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| Order / More Info | DNA Polymerase I Klenow Fragment, Exo-Minus, Recombinant from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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