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| Product Name | Sirt1, Fluorogenic Assay Kit, BioAssay™ (NAD-dependent Deacetylase Sirtuin-1, hSIRT1, SIR2-like Protein 1, hSIR2, SIR2L1) |
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| Description | The Fluorogenic SIRT1 Assay Kit is a complete assay system designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT1 (Sir2) activity measurements. In addition, the kit includes purified SIRT1 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT1 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with a sample containing SIRT1 activity (e.g. HeLa nuclear extract or purified SIRT1 enzyme). The deacetylation sensitizes the s |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 137369 |
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| Order / More Info | Sirt1, Fluorogenic Assay Kit, BioAssay™ (NAD-dependent Deacetylase Sirtuin-1, hSIRT1, SIR2-like Protein 1, hSIR2, SIR2L1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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