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| Product Name | MEK1 Total, l Sandwich ELISA Kit, BioAssay™ (Map Kinase Kinase, MAPK Kinase, Erk Kinase 1) |
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| Description | Detects endogenous levels of total MEK1 protein. A significant induction of MEK1 phosphorylation can be detected in PMA-treated NIH/3T3 cells using Phospho- MEK1 (Ser217/221) Sandwich ELISA Kit. However, the level of total MEK1 detected by this sandwich ELISA kit remains unchanged. This kit can also be used to detect total MEK1 protein in human 293 cells. Total MEK1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total MEK1 protein. MEK1 Mouse mAb has been coated onto the microwells. After incubation with cell lysates, total MEK1 protein (phospho- and nonphospho-) is captured by the coated antibody. Following extensive washing, MEK1/2 Antibody is added to detect the captured MEK1 protein. HRP-linked anti-rabbit antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of t |
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| Size | 1 kit |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | M2865-12A |
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| Order / More Info | MEK1 Total, l Sandwich ELISA Kit, BioAssay™ (Map Kinase Kinase, MAPK Kinase, Erk Kinase 1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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