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| Product Name | Phosphoinositide 3 Kinase, p85 alpha, p110 delta, Recombinant, Human, GST-Tag (PI3K) |
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| Description | Purity ~50% by SDS-PAGE. Complex of N-terminal GST tagged recombinant full-length human p110d ( GenBank Accession No. NM_005026), and recombinant full length, human p85a (GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 3nmol/mg/min using phosphatidylinositol as the substrate. p110d/p85a activity was determined using PIP2 and ATP as substrates. Assay Conditions: Enzyme reaction was carried out for 15 minutes at 30°C using 5-200ng of P4073-30B1 in a buffer containing 40mM Tris-HCl, pH 7.5, 20mM magnesium chloride, 100um ATP, 200uM L-alpha-phosphatidylinositol/L-alpha-phosphatidylserine (PI/PS) substrate. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
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| Size | 20ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: NM_005026 |
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| Catalog # | P4073-30B1 |
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| Order / More Info | Phosphoinositide 3 Kinase, p85 alpha, p110 delta, Recombinant, Human, GST-Tag (PI3K) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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