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| Product Name | Caspase-4 Fluorometric BioAssay™ Kit |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric BioAssay™ Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. Sample Type: Cell and tissue lysates Intended Use: For use in detecting the early/middle stages of apoptosis and differentiating apoptosis from necrosis. Specificity: Recognizes Caspase-4. Species Crossreactivity: mammalian Test Principle: The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (400nm); upon cleavage of the substrate by caspase-4 or related caspases, free AFC emits a yellow-green fluorescence (505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity. Kit |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 219570 |
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| Order / More Info | Caspase-4 Fluorometric BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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