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| Product Name | Caspase-1 Fluorometric BioAssay™ Kit (ICE) |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Fluorometric BioAssay™ Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. Sample Type: Cell and tissue lysates Intended Use: For use in detecting the early/middle stages of apoptosis and to differentiate apoptosis from necrosis. Specificity: Recognizes Caspase-1. Species Crossreactivity: mammalian Test Principle: The assay is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). YVAD-AFC emits blue light (400nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 219533 |
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| Order / More Info | Caspase-1 Fluorometric BioAssay™ Kit (ICE) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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