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| Product Name | Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1) |
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| Description | Purity ≥ 98% (HPLC). Fluorometric substrate for Caspase-3. N-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Used in the BioAssay™ Caspase-3 Apoptosis Detection Kit (Cat.# C2087-10), providing a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7uM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC (7-amino-4-trifluoromethyl coumarin) at Ex 400nm and Em 505nm, using a fluorometer or a multi-well fluorescence plate reader. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increas |
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| Size | 200 tests, 1000 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | N-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2087-11 |
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| Order / More Info | Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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