| 产品详情 |
| Edit |   |
|---|
| Product Name | NNMT Inhibitor Screening Assay Kit (Fluorometric) |
|---|
| Description | N'-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. The NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT. |
|---|
| Size | n/a |
|---|
| Concentration | n/a |
|---|
| Applications | Measurement of Beta-Galactosidase activity in various samples |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | LS-K712 |
|---|
| Price | |
|---|
| Order / More Info | NNMT Inhibitor Screening Assay Kit (Fluorometric) from LIFESPAN BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|