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| Product Name | Macrophage Colony Stimulating Factor, Human (M-CSF), BioAssay™ ELISpot Kit |
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| Description | Intended Use: This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human M-CSF. Principle: Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human M-CSF binds to antibody coated microtiter plate. Microtiter plate coated with human M-CSF Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human M-CSF. Substrate Solution is added. A colored precipitate forms and appears as spots at the sites of human M-CSF secreting location. Each individual spot representing an individual human M-CSF secreting cell. Background: Human M-CSF, also known as CSF-1, can be either expressed on the cell surface as a membrane-spanning 68-86 kD chondroitin or secreted as an 80-100 kD glycop |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | M1201-07A |
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| Order / More Info | Macrophage Colony Stimulating Factor, Human (M-CSF), BioAssay™ ELISpot Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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